EDT - Electron Density Topography
About EDT
EDT is the integration of accurate and precise solution scattering measurements up to the middle angle region, electron density calculations utilizing DENSS [1, 2] and intelligent routines for evaluation of self-consistency of the data and results, including a machine learning process for classification of results to cope with the many-solution and many-body problems in the process [3].
Electron density of a monoclonal antibody revealed by EDT
Advertisement feature For protein imaging, the answer is in solution
Feature article produced by Nature Research Custom Media and Rigaku.
The observation of intact protein structures in solution are almost impossible to capture with current technology. Is that about to change?
(An image of bovine superoxide dismutase obtained in a solution using EDT technology is shown.)
Publications and resources
- Grant, T. D. (2018). Ab initio electron density determination directly from solution scattering data. Nature Methods, 15(3), 191-193. (https://doi.org/10.1038/nmeth.4581)
- Grant, T. D. (2022). Reconstruction of 3D density from solution scattering. Methods in Enzymology, Volume 678, 145-186. (https://doi.org/10.1016%2Fbs.mie.2022.09.018)
- Takashi M., Akihito Y., Takashi S. (2023). 3D molecular visualization of a human antibody by MAXS measurement reveals significant differences between the solution and crystalline states. Rigaku Journal, 39(1).
Our presentations
- Dec 2024: Antibody Engineering & Therapeutics, San Diego, USA
- Dec 2024: The Antibody Society of Japan, Sendai, Japan
- Nov 2024: Protein Sciences in Drug Discovery, Cambridgeshire, UK
- Nov 2024: Japanese Biochemical Society, Yokohama, Japan
- Nov 2024: Protein Structure Determination in Industry, Paris, France
- Oct 2024: Chem-Bio Informatics Society, Tokyo, Japan
- Oct 2024: Antibody Engineering & Therapeutics Asia, Kyoto, Japan
- Sep 2024: The Society for Biotechnology, Tokyo, Japan
- Sep 2024: CASSS/Higher Order Structure Symposium, Rockville, USA
- Jun 2024: The Protein Society, Vancouver, Canada
- Jun 2024: Protein Science Society of Japan, Sapporo, Japan
- Mar 2024: Pharmaceutical Society of Japan, Yokohama, Japan
- Dec 2023: Antibody Engineering & Therapeutics, San Diego, USA
- Dec 2023: The Antibody Society of Japan, Kagoshima, Japan
- Oct 2023: Antibody Engineering & Therapeutics Digital week
- Sep 2023: The Society for Biotechnology, Nagoya, Japan
- May 2023: Antibody Engineering & Therapeutics Digital week
- Mar 2023: Bispecific Antibodies Asia & Pacific